THE BRANTFORD STUDIES
In the city of Brantford, Canada, two independent trials were conducted.
In this study no control procedure was attempted. However, it will be considered briefly because two pre-fluoridation surveys were made by the school dental officer and his assistant (Hutton et al., 1954). This is the only one of these five trials in which more than one pre-fluoridation survey was made in the test city; and, with the exception of Muskegon, none of the control cities provided data obtained in successive years from individual yearly age groups.
Were results combined or averaged? Hutton et al. (1951) stated that "The results of these two [pre-fluoridation] surveys have been combined and are shown in Table I." In Tables I and II of the Ontario Department of Health Report (1956) the rates for those two surveys were shown separately. With the exception of those of the nine-year-old children, for both the deciduous and the permanent teeth, the mean of the two rates for each age is identical (to one decimal place) with the mean rate computed from the figures of the combined survey which were supplied by the authors (Hutton et al., 1951, Table I). This result could have arisen only if (with the exception of the children who were nine years old) the number of children of the same age examined on both occasions was equal, or almost exactly so - a most unlikely event; or if the results were not combined, as stated by the authors, but the rates obtained in 1944 and 1945 were averaged. The United Kingdom Mission (1953) stated that "the average figures of these two years" were used. If the rates for the two years were averaged, there were errors in computing the rates of the nine-year-old children, or errata in one or more of those three tables. The figures shown in Tables I and II of the Ontario Department of Health Report (1956) should be treated with caution, because in both of these the year of fluoridation is stated incorrectly, and in the former table the "% Reduction Since 1944-45" for age seven years should be 66, not 51; whereas in the latter one, the "% Reduction Since 1948" for age nine to eleven years in Stratford and Sarnia should not have been indicated by dashes, but by five and sixteen respectively.
The other study in Brantford was conducted by the Canadian Department of National Health and Welfare, and was described by the New Zealand Commission of Inquiry (1957) as "the most complete of the 10-year North American studies".
Late commencement. Unfortunately, this trial was not begun until January 1948, over two and a half years after the commencement of fluoridation of the Brantford water supply (Brown, 1951). Such delay must affect the value of this study, unless it is assumed that the structural theory of reduction of dental caries as a result of the ingestion of fluorides is correct, and that this is the only way in which fluorides may affect the incidence of caries. This theory was advanced by Cox and Levin in 1942, and was widely accepted at the time these trials were initiated (Dean et al., 1950; Ast et al., 1950). If this theory is correct (as was noted in Part One), little change can be expected in the DMF rates until about six years after the commencement of the study. It is evident that this theory must still be held to be correct in some quarters, for the recent report of a dental caries survey conducted by McCauley and Frazier (1957) stated: "Although fluoridation of the Baltimore City water supply was begun Nov. 26, 1952, (27 months before the survey), there was no reason to anticipate substantial change in the caries experience of these children in this relatively short period of time." However, even before the commencement of the Department of National Health and Welfare study in Brantford, the City Health Department examiner's figures for 1947 showed great reductions in the DMF rates since the introduction of fluoridation. This result was not published by Hutton et al. until 1951, but must have been available to the investigators who "came to the scientific rescue of the project early in 1948" (Hutton et al., 1956). Indeed, in his first report, Brown (195 1) acknowledged the help and advice of two of the three authors of the City Health Department Report (Hutton et al., 1951).
The control cities. The city of Sarnia was chosen as the "fluoride-free" control, and Stratford as the control city with a water supply which "contains 1.3 ppm. of fluorine from a natural source" Brown, 1951). The reasons for the selection of these cities were not given, except that it was said: "Sarnia and Stratford, two cities in Western Ontario known to be comparable to Brantford, except for the fluoride content of their water supplies, agreed to serve as controls" (Brown et at.\,1954b). Also, Brown, Josie and Stewart (1953) said that Sarnia was "a city" which has fluoride-free water and is sufficiently similar in size, location, and other attributes for purposes of the comparison". The United Kingdom Mission (1953) stated: "Before this study was undertaken the socio-economic status of the three communities was examined and found to be reasonably comparable."
Superior dental care in Brantford. The United Kingdom Mission (1953) said: "Brantford, however, over a period of 15 years, has provided more free dental services for children than most Canadian cities, and this has resulted in the ratio of corrected to total defects being higher than in either Sarnia or Stratford." It considered that in Brantford "dental care was outstandingly good." Also, Brown, in 1952, said: "the recordings so far obtained indicate both a higher treatment and an apparently better oral hygiene status of the Brantford children when compared with the controls, and it is therefore suggested that caution should be exercised in the interpretation of the rates shown. The lack of a pre-fluoridation survey on a comparable basis is a further limiting factor in interpreting the results."
No pre-fluoridation survey. The authors of this Brantford study (Brown et al., 1953) said: "As the study does not include a pre-fluoridation survey, the full amount of benefit which the Brantford teeth have received since fluoridation cannot be illustrated directly from the data for Brantford. Some idea of the extent of the benefit can be obtained by comparison with the data for Sarnia.... By 1948 the Brantford data were not greatly different from those for Sarnia."
This remark suggests that the data for the two cities prior to fluoridation in Brantford were similar, and that this process had had little effect on the caries rates up to the time of the 1948 examination in Brantford.
Doubtful comparability of rates. Owing to the delay in setting up this study, it cannot be established how closely the dental caries attack rates in Brantford resembled those in Sarnia, at the time fluoridation was instituted in the former city. There is evidence that the dental condition of the children in those two cities was not closely comparable, for Brown et al. (1953) stated that "even by the time of the first survey, mean tooth mortality in Brantford was much lower than in Sarnia, for all age groups."
This comment implies that, even by the time of the first survey, as a result of fluoridation the tooth mortality in Brantford had decreased considerably. This concept is not consistent with the one mentioned in the last paragraph. At the time of the first examinations, the tooth mortality in the six to eight years age group was more than four times as great in Sarnia as it was in Brantford, and in each of the other two age groups it was almost twice as great (Brown et al., 1953, Table 3.).
The gross differences observed in the tooth mortality (teeth which are missing or which must be extracted) in Brantford and its control city of Sarnia, during the initial examinations.
Canadian Department of Health and Welfare study.
The influence of treatment.The fact that such large differences were reported in tooth mortality rates in the two cities even in the older age groups suggests that dental treatment in them was different, and the authors stated that "Both preventive and treatment measures may have a decided effect on tooth mortality rates" (Brown et al., 1953). It may be recalled that the United Kingdom Mission (1953) noted that Brantford was unusually well provided with free dental services "and this has resulted in the ratio of corrected to total defects being higher than in either Sarnia or Stratford."
Tooth mortality. Brown et al. (1953) said that "there has been a decrease in tooth mortality in Brantford between successive surveys"; but, in fact, the 1953 rate (Table 3) in the children aged six to eight years was the highest up to that time. This statement was corrected in the next report (Brown et al., 1954b) by prefixing "in almost all cases" to the previous statement. In this connection, the authors remarked in 1953 that, "as well as the fluoridation of the Brantford-water supply, other factors such as differences in preventive or treatment measures are probably affecting the Brantford position." There appears to be no reason why those of the control cities should not have been similarly affected.
Differences in oral hygiene. Additional evidence which suggests that a difference existed between the dental condition of the children in Brantford and that of children in the control cities is provided by the data with regard to oral hygiene. Brown et al. (1954b) stated: "Classification and recording of oral hygiene was undertaken because it was considered that marked differences in oral hygiene as between the test and control groups might conceivably affect the findings - or at least might be taken into consideration as a modifying factor, although not a strictly measurable one. However, the figures here suggest that, since 1948, differences in oral hygiene status could not have been a major factor in either the caries level changes within Brantford or the caries level differences between the control cities."
As no comparisons were made between the control cities, the last phrase of the quotation is thought to refer to the caries level differences between Brantford and each of the control cities. The authors' Table 11 indicates that, in the first examinations, in the test city the percentage of subjects who had a good oral hygiene status was almost twice as great as that present in children in both the control ones; these were, Brantford 34.3 per cent, Sarnia 19.7 per cent and Stratford 17.8 per cent. Considerable differences between the oral hygiene status of the children in the test and the control cities were also recorded during the later examinations. These were clearly "marked differences" though the authors did not consider them important.
The concentration of fluorides. Brown et al. (1954b) stated: "The Brantford Fluoridation Caries Study was undertaken with a view to finding out whether or not the raising of the fluoride content of a previously fluoride-free water supply to 1 part per million, by the mechanical addition of sodium fluoride, would reduce the incidence of dental caries to that which obtains where water supplies derive about 1 part per million of fluoride from deposits in the earth."
A fundamental requirement of a test of this nature is that the water supply of the control city should contain the same concentration of fluorides as that of the test one, but Brown, McLaren, Josie and Stewart (1956) reported: "The Stratford water supply contains a concentration of fluoride which is 60% higher than that used in Brantford."
Differences of opinion. Several different statements were made regarding the concentrations of fluorides which were present in the water supplies of Brantford and Stratford.
1. Brantford. The New Zealand Commission of Inquiry (1957) said that the water supply of Brantford was "raised to 1.2 ppm. in 1945". The authors of the City Health Department study (Hutton et al., 1951) stated that in February 1949, "the dosage was raised to produce 1.20 ppm."; but in 1954 they stated that "The fluoride content of the finished water is maintained at 1 ppm." In reporting the National Health and Welfare Study, Brown (1952) stated that "a fluorine concentration of between 1.0 and 1.2 ppm. has been maintained in the water supply continuously" since June 1945; and in 1956 Brown et al. said: "Brantford has had more than 10 years of experience with 1 part per million fluoride in its water supply." These statements that the fluoride content was "maintained" at "1 ppm" and "between 1.0 and 1.2 ppm" should be considered in conjunction with that of the United Kingdom Mission (1953): "For example, in 1951 the average for the year was 1.2 ppm with a variation between 0.75 and 1.45 ppm., however, the figures below 1.1 ppm and above 1. 3 ppm were few in number."2. Stratford. The supply of Stratford was stated to have 1.2 ppm of natural fluoride in its domestic water (Ontario Department of Health, 1956; New Zealand Commission of Inquiry, 1957). However, in reporting the National Health and Welfare study in 1951 and 1952, Brown stated that it "contains 1.3 ppm. of fluorine"; and, with his co-workers, the following year said that "in Stratford where the water supply, obtained from deep wells, has contained 1.3 ppm." (fluoride) "from natural deposits continuously since 1917" (Brown et al., 1953). In their next report dealing with dental caries (Brown et al., 1954b), the figure stated was "1.3 to 1.6 parts per million of fluoride"; but in the following report these authors (Brown et al., 1956) said that this water supply "contained 1.6 parts per million of fluoride since 1917", and also that 1.6 ppm fluoride content "has been in continuous use for thirt yeight years."
These different statements, although strange, may be considered to be unimportant from the practical point of view, but very small changes in the fluoride content of the water may have considerable effects, as reports by Deatherage (1942) and Galagan and Lamson (1953) indicated. The latter authors found that "In water supplies of the Arizona communities studied, concentrations of fluoride above 0.8 ppm resulted in objectionable dental fluorosis; concentrations of 0.6 to 0.8 ppm resulted in an occasional diagnosis of fluorosis; concentrations below 0.6 ppm did not cause objectionable fluorosis." The mean temperatures of these communities were between 67 and 72 degrees Fahrenheit.
Three misleading statements. In the summary of the 1954b report of the National Health and Welfare study, Brown et al. stated that during the period 1948-54 "dental caries experience of children in the two control cities, on the other hand, either has remained at about the 1948 levels, or has increased slightly, at all ages studied." A similar statement was made by them in the 1955 Report (Brown et al., 1956): "During that time [more than ten years] a very important, statistically significant reduction in tooth decay has occurred in all the age groups studied, while in the two control cities of Sarnia and Stratford it has either remained at about the same level or increased somewhat." The last sentence contains three misleading statements about the control cities:
(a) As this study did not commence until 1948 with "examinations in Sarnia beginning in March of that year, and in Stratford in October" (Brown, 1952), and as fluoridation in Brantford commenced in June 1945 (Hutton et al., 195 1), no information is available with regard to the prevalence of "tooth decay" in Sarnia during the first two and three-quarter years, or in Stratford for the first three and a quarter years of the ten-year period of fluoridation which these authors were discussing in their 1955 Report. Therefore, it cannot be known whether this condition "remained at about the same level" in the control cities during the early years of fluoridation in Brantford.
(b) No remarks were made in the context of this statement in this (or the previous) paper which suggested that reference was being made to the permanent teeth only, but these statements are not correct for the deciduous teeth. (However, in the former paper, a similar statement to that made in the summary was also made under the heading "Mean DMF Permanent Teeth".) Decreases in the df (decayed, filled deciduous teeth) rate were seen in the nine to eleven years group in both Sarnia and Stratford, that in the former city being shown by these authors to be statistically significant (Brown et al., 1954b, Table 10).
(c) In both cities the mean rates of DMF and df teeth per child showed changes which were said to be statistically significant (Brown et al., 1954b).
Omission of decreases. The decreases in the caries attack rates of the deciduous teeth, which have been mentioned above, were also omitted in Table II of the Report to the Minister of Health, Province of Ontario, Canada, by the Division of Medical Statistics, Ontario Department of Health, which was made in 1955 (Ontario Department of Health, 1956). Under the heading "% Reduction Since 1948", these decreases were not shown, but instead, in the appropriate positions dashes were printed, despite the fact that in Sarnia the percentage reduction (determined by the method commonly used in these studies) was 16 per cent, almost as great as that of 18 per cent shown for the same age group in the test city; furthermore, this reduction in Sarnia was stated by the authors (Brown et al., 1954b) to be statistically significant.
Different rates reported. It should be noted that the deficiency in the data of the National Health and Welfare study, owing to its late commencement, could not be decreased by comparing the rates obtained by its examiners with those reported by the City Health Department examiner, because of the considerably lower rates recorded by the last-mentioned examiner when impecting similar groups of children. For instance, in their examination in 1948, for children aged six to eight, nine to eleven and twelve to fourteen years, Brown et al. (1953) obtained rates of 1.41, 4.07 and 7.68 respectively for the permanent teeth, compared with rates of 0.84, 3.37 and 6.11, for the same age groups of children, in the same city and in the same year, obtained by the City Health Department examiner (calculated from Table IV, Hutton et al., 1951).
Significant fluctuations in controls. In the two control cities "where it is presumed that there has been no appreciable change in either preventive or treatment services" (United Kingdom Mission Report; 1953), it can be seen in Tables 4, 6, 8 and 10 published by Brown et al. (1954b) that some considerable fluctuations in the caries attack rates were recorded; more than half of the inter-year differences in each of the control cities being shown to be statistically significant. However, in the text it was stated that the "dental caries experience of children in the two control cities ... either has remained at about the 1948 levels, or has increased slightly, at all ages studied."
(1) Sarnia. In this city the changes between examinations of the rates for the deciduous teeth were not very marked, but there was a significant one between 1948 and 1954 in the nine to eleven years age group. However, in the DMF permanent teeth, there were four definitely significant (three standard error level) and one significant change in the nine comparisons made. In regard to the first permanent molars, there were six significant (including three definitely significant) alterations in the rates, in the nine comparisons made (Brown et al., 1954b).
(2) Stratford. In this city, the rate of df teeth per child showed a significant difference in one case out of the four comparisons made between successive examinations (Brown et al., 1954b). In the DMF permanent teeth per child, the results of the four examinations were: 0.41, 0.75, 0.47 and 0.67 for the six to eight years group; 1.13, 1.76, 1.46 and 1.89 for the nine to eleven years age group; 2.55, 3.12, 3.02 and 3.77 for the twelve to fourteen years age group (Brown et al., 1954b, 1956). These variations between examinations were so large that five out of the six comparisons made (in the 1954b report) between successive examinations were said to be statistically significant, four of them being at the three standard error level. In the last report published (1956), Brown et al. abandoned the method which they had used in the two previous ones, that of showing the standard error of the mean values of the DMF rates, and of making "Inter-City" and "Inter-Year" comparisons (Brown et al., 1953, 1954b). Therefore it was not stated whether the differences between the 1954 and the 1955 DMF rates in Stratford were significant, but it can be seen that they were marked; the difference of 0.75 in the twelve to fourteen years group being considerably larger than any of those stated in the 1954b report to be significant differences between various examinations in that city. When the DMF rates for the first permanent molars are considered, similar marked changes are seen, and in five of the six comparisons the differences were significant (four definitely so).
Larger "percentage" changes in a control. If one resorts to the method commonly used in these trials - that of expressing the alteration in the DMF rate as a percentage of the original rate - these unexplained increases in the control city of Stratford between 1948 and 1955, although they were described as "no change" (Ontario Department of Health, 1956), and as "a slightly higher prevalence of dental caries in 1955, over the 1948 levels" (Brown et al., 1956), are found to be 63 per cent, 67 per cent and 48 per cent, for the six to eight, nine to eleven and twelve to fourteen years age groups respectively. In each case these percentage changes are considerably larger than those of 51 per cent, 44 per cent and 37 per cent which can be computed from the data reported for Brantford. The last-mentioned changes were attributed to fluoridation, and each was stated to indicate "a very important, statistically significant reduction in tooth decay" (Brown et al., 1956).
This is just one instance of the strange results which are obtained when this method of calculation is used. It should be realized that it was the one most commonly employed in fluoridation trials, and was used in formulating the often-expressed claim that (as stated by Arnold et al., 1956): "In children born since fluoridation was put into effect, the caries rate for the permanent teeth was reduced on the average by about 60 per cent." The recent World Health Organization Press Release (WHO/45, 4 September 1957) stated - with no mention of age - "The prevalence of dental caries in the permanent teeth of children decreased some 60 percent".
A smaller "percentage decrease" after long fluoridation. The "percentage decreases" which have just been mentioned (51 per cent, 44 per cent and 37 per cent, calculated by the method described in the last paragraph) were not stated in the 1956 report of Brown et al., but the figure of 51 per cent for the six to eight years age group is considerably less impressive than the figure of "approximately 69%" published in the 1954b report from this study. Although the final report (1956) gave the rates for 1948 and 1955 only, and therefore did not show the fluctuations between examinations, from the 1954b and 1956 reports of Brown et al. it is seen that the marked change in the "percentage" decrease which has just been mentioned was due to the DMF rate in Brantford in 1955, for this age group, being the highest seen since 1951. Ignoring the fact that in "children born subsequent to fluoridation" the "decrease" in the DMF rate had dropped to only 51 per cent, the authors stated in the final sentence of their final report (1956): "For every three decayed teeth they would have had, they have only one."
More misleading comments. Turning from the reports made by the authors of this study about the control cities to some of the comments made by others, it is seen that these are even more misleading. Only two will be mentioned. Martin (1956) stated that during "the 1948-54 period" the "DMF figures for the two control areas have remained at 1948 levels." The authors of the Ontario Department of Health Report (1956) went so far as to state to their Minister of Health that "it had been established that there has been no change in the already low dental caries attack rates in Stratford ... or in the relatively high rates for Sarnia".
These two statements are contrary to the results published by the authors of the study (Brown et al., 1954b), which showed that in both the control cities there were statistically significant differences between the caries attack rates at successive examinations. Out of the fifteen comparisons made, only five differences in the rates were not significant, two changes were significant and eight changes were definitely significant.
Unexplained significant changes in controls. All the changes in the caries attack rates in the control cities which were reported to be significant are unlikely to be chance variations; therefore, to what factor or factors must they be attributed. It is possible that they were due, in whole or in part, to alterations in the "weighting", such as were found in the Evanston study as a result of combining the caries attack rates of children of different ages (Hill et al., 1952). However, as the age composition of the groups was not stated in this study, it cannot be determined to what degree the data was distorted by "weighting", a condition which is almost inevitably present when data drawn from several different yearly age groups are combined.
Apart from deficiencies which are found in other studies also, in this trial there is an absence of any information regarding the caries attack rates in Brantford and Sarnia, prior to the fluoridation of the water supply of the former city. There is also the fact that no explanation was given by the authors for the significant variations in the caries rates in the control areas. Therefore, a marked decrease in dental caries in the test city as a result of fluoridation cannot be said to have been established.